|
KMID : 1140420230320030247
|
|
Journal of Obesity & Metabolic Syndrome
2023 Volume.32 No. 3 p.247 ~ p.258
|
|
|
Herbal Metabolites as Potential Carbonic Anhydrase Inhibitors: Promising Compounds for Cancer and Metabolic Disorders
|
|
Ebrahim Yarmohammadi
Lee Sang-Hun
Zahra Khamverdi
Marzieh Savari
Amir Taherkhani
|
|
|
Abstract
|
|
|
Background: Human carbonic anhydrases (CAs) play a role in various pathological mechanisms by controlling intracellular and extracellular pH balance. Irregular expression and function of CAs have been associated with multiple human diseases, such as obesity, cancer, glaucoma, and epilepsy. In this work, we identify herbal compounds that are potential inhibitors of CA VI.
Methods: We used the AutoDock tool to evaluate binding affinity between the CA VI active site and 79 metabolites derived from flavonoids, anthraquinones, or cinnamic acids. Compounds ranked at the top were chosen for molecular dynamics (MD) simulations. Interactions between the best CA VI inhibitors and residues within the CA VI active site were examined before and after MD analysis. Additionally, the effects of the most potent CA VI inhibitor on cell viability were ascertained in vitro through the 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
Results: Kaempferol 3-rutinoside-4-glucoside, orientin, kaempferol 3-rutinoside-7-sophoroside, cynarin, and chlorogenic acid were estimated to establish binding with the CA VI catalytic domain at the picomolar scale. The range of root mean square deviations for CA VI complexes with kaempferol 3-rutinoside-4-glucoside, aloe-emodin 8-glucoside, and cynarin was 1.37 to 2.05, 1.25 to 1.85, and 1.07 to 1.54 A, respectively. The MTT assay results demonstrated that cynarin had a substantial effect on HCT-116 cell viability.
Conclusion: This study identified several herbal compounds that could be potential drug candidates for inhibiting CA VI.
|
|
|
KEYWORD
|
|
|
Anthraquinones, Neoplasms, Carbonic anhydrases, Cinnamic acid, Flavonoids, Inhibitor, Obesity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|